Research

Author

Anthony Chan

Published

2024-08-24

Overview

Functional Genomics, Cancer Biology, Drug Discovery

Selected Publications (7)

Reverse Chronological Order; *First/Co-first Authorship (3); **Second Authorship (3)

  1. Mattson, N.M., Chan, A.K.N.**, Miyashita, K., Mukhaleva, E., Chang, W.-H., Yang, L., Ma, N., Wang, Y., Pokharel, S.P., Li, M., et al. (2024). A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling-instructed genetic screens. Nat. Struct. Mol. Biol. 31, 465–475. 10.1038/s41594-024-01211-y. Highlighted in numerous news sources (link).
  1. Chan, A.K.N.*, Han, L., Delaney, C.D., Wang, X., Mukhaleva, E., Li, M., Yang, L., Pokharel, S.P., Mattson, N., Garcia, M., et al. (2024). Therapeutic targeting Tudor domains in leukemia via CRISPR-Scan Assisted Drug Discovery. Sci. Adv. 10, eadk3127. 10.1126/sciadv.adk3127.
  1. Li, M., Yang, L., Chan, A.K.N., Pokharel, S.P., Liu, Q., Mattson, N., Xu, X., Chang, W.-H., Miyashita, K., Singh, P., et al. (2023). Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Adv. Sci. 10, 2206584. 10.1002/advs.202206584.
  1. Xu, X., Chan, A.K.N.**, Li, M., Liu, Q., Mattson, N., Pangeni Pokharel, S., Chang, W.-H., Yuan, Y.-C., Wang, J., Moore, R.E., et al. (2022). ACTR5 controls CDKN2A and tumor progression in an INO80-independent manner. Sci. Adv. 8, eadc8911. 10.1126/sciadv.adc8911.
  1. Liu, Q., Chan, A.K.N.**, Chang, W.-H., Yang, L., Pokharel, S.P., Miyashita, K., Mattson, N., Xu, X., Li, M., Lu, W., et al. (2022). 3-Ketodihydrosphingosine reductase maintains ER homeostasis and unfolded protein response in leukemia. Leukemia 36, 100–110. 10.1038/s41375-021-01378-z.
  1. Yang, L.*, Chan, A.K.N.*, Miyashita, K.*, Delaney, C.D., Wang, X., Li, H., Pokharel, S.P., Li, S., Li, M., Xu, X., et al. (2021). High-resolution characterization of gene function using single-cell CRISPR tiling screen. Nat. Commun. 12, 4063. 10.1038/s41467-021-24324-0. Featured by 10x Genomics (link).
  1. Chan, A.K.N.*, and Chen, C.-W. (2019). Rewiring the Epigenetic Networks in MLL-Rearranged Leukemias: Epigenetic Dysregulation and Pharmacological Interventions. Front. Cell Dev. Biol. 7. 10.3389/fcell.2019.00081.

All Publications (23)

Reverse Chronological Order; *First/Co-first Authorship (6); **Second Authorship (3)

  1. Mattson, N.M., Chan, A.K.N.**, Miyashita, K., Mukhaleva, E., Chang, W.-H., Yang, L., Ma, N., Wang, Y., Pokharel, S.P., Li, M., et al. (2024). A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling-instructed genetic screens. Nat. Struct. Mol. Biol. 31, 465–475. 10.1038/s41594-024-01211-y. Highlighted in numerous news sources (link).
  1. Chan, A.K.N.*, Han, L., Delaney, C.D., Wang, X., Mukhaleva, E., Li, M., Yang, L., Pokharel, S.P., Mattson, N., Garcia, M., et al. (2024). Therapeutic targeting Tudor domains in leukemia via CRISPR-Scan Assisted Drug Discovery. Sci. Adv. 10, eadk3127. 10.1126/sciadv.adk3127.
  1. Xue, M., Dong, L., Zhang, H., Li, Y., Qiu, K., Zhao, Z., Gao, M., Han, L., Chan, A.K.N., Li, W., et al. (2024). METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation. J. Hematol. Oncol. 17, 7. 10.1186/s13045-024-01526-9.
  1. Schiedel, M., McArdle, D.J.B., Padalino, G., Chan, A.K.N., Forde-Thomas, J., McDonough, M., Whiteland, H., Beckmann, M., Cookson, R., Hoffmann, K.F., et al. (2023). Small Molecule Ligands of the BET-like Bromodomain, SmBRD3, Affect Schistosoma mansoni Survival, Oviposition, and Development. J. Med. Chem. 66, 15801–15822. 10.1021/acs.jmedchem.3c01321.
  1. Kumar, A., Khani, A.T., Duault, C., Aramburo, S., Ortiz, A.S., Lee, S.J., Chan, A., McDonald, T., Huang, M., Lacayo, N.J., et al. (2023). Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemia. J. Immunother. Cancer. 11, e006649. 10.1136/jitc-2022-006649.
  1. Li, M., Yang, L., Chan, A.K.N., Pokharel, S.P., Liu, Q., Mattson, N., Xu, X., Chang, W.-H., Miyashita, K., Singh, P., et al. (2023). Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis. Adv. Sci. 10, 2206584. 10.1002/advs.202206584.
  1. Xu, X., Chan, A.K.N.**, Li, M., Liu, Q., Mattson, N., Pangeni Pokharel, S., Chang, W.-H., Yuan, Y.-C., Wang, J., Moore, R.E., et al. (2022). ACTR5 controls CDKN2A and tumor progression in an INO80-independent manner. Sci. Adv. 8, eadc8911. 10.1126/sciadv.adc8911.
  1. Sellar, R.S., Sperling, A.S., Słabicki, M., Gasser, J.A., McConkey, M.E., Donovan, K.A., Mageed, N., Adams, D.N., Zou, C., Miller, P.G., …Chan, A., et al. (2022). Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells. J. Clin. Invest. 132. 10.1172/JCI153514.
  1. Liu, Q., Chan, A.K.N.**, Chang, W.-H., Yang, L., Pokharel, S.P., Miyashita, K., Mattson, N., Xu, X., Li, M., Lu, W., et al. (2022). 3-Ketodihydrosphingosine reductase maintains ER homeostasis and unfolded protein response in leukemia. Leukemia 36, 100–110. 10.1038/s41375-021-01378-z.
  1. Kumar, A., Lee, S.J., Liu, Q., Chan, A.K.N., Pokharel, S.P., Yu, J., Chen, C.-W., and Swaminathan, S. (2021). Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc. 2, 100874. 10.1016/j.xpro.2021.100874.
  1. Laurin, C.M.C., Bluck, J.P., Chan, A.K.N., Keller, M., Boczek, A., Scorah, A.R., See, K.F.L., Jennings, L.E., Hewings, D.S., Woodhouse, F., et al. (2021). Fragment-Based Identification of Ligands for Bromodomain-Containing Factor 3 of Trypanosoma cruzi. ACS Infect. Dis. 7, 2238–2249. 10.1021/acsinfecdis.0c00618.
  1. Brand, M., Clayton, J., Moroglu, M., Schiedel, M., Picaud, S., Bluck, J.P., Skwarska, A., Bolland, H., Chan, A.K.N., Laurin, C.M.C., et al. (2021). Controlling Intramolecular Interactions in the Design of Selective, High-Affinity Ligands for the CREBBP Bromodomain. J. Med. Chem. 64, 10102–10123. 10.1021/acs.jmedchem.1c00348.
  1. Yang, L.*, Chan, A.K.N.*, Miyashita, K.*, Delaney, C.D., Wang, X., Li, H., Pokharel, S.P., Li, S., Li, M., Xu, X., et al. (2021). High-resolution characterization of gene function using single-cell CRISPR tiling screen. Nat. Commun. 12, 4063. 10.1038/s41467-021-24324-0. Featured by 10x Genomics (link).
  1. Siddique, F., Lam, C.C., Ng, K.L., Chan, K.N.A., and Wong, W.K. (2021). Characterization and expression of a cellobiase Cba2 of Cellulomonas biazotea: The first bacterial β-glucosidase demonstrated to possess a genuine signal peptide. Res. J. Biotechnol. 16. link.
  1. Lo, H.S., Hui, K.P.Y., Lai, H.-M., He, X., Khan, K.S., Kaur, S., Huang, J., Li, Z., Chan, A.K.N., Cheung, H.H.-Y., et al. (2021). Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir. ACS Cent. Sci. 7, 792–802. 10.1021/acscentsci.0c01186. Highlighted in numerous news sources (link).
  1. Chan, A.K.N.*, and Chen, C.-W. (2019). Rewiring the Epigenetic Networks in MLL-Rearranged Leukemias: Epigenetic Dysregulation and Pharmacological Interventions. Front. Cell Dev. Biol. 7. 10.3389/fcell.2019.00081.
  1. Chan, A.K.N.*, Ng, A.K.L., Ng, K.K.Y., and Wong, W.K.R. (2018). Cloning and characterization of two novel β-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea. Gene 642, 367–375. 10.1016/j.gene.2017.11.043.
  1. Pliotas, C.*, Grayer, S.C.*, Ekkerman, S.*, Chan, A.K.N.*, Healy, J., Marius, P., Bartlett, W., Khan, A., Cortopassi, W.A., Chandler, S.A., et al. (2017). Adenosine Monophosphate Binding Stabilizes the KTN Domain of the Shewanella denitrificans Kef Potassium Efflux System. Biochemistry 56, 4219–4234. 10.1021/acs.biochem.7b00300.
  1. Ng, W.-L., Li, H.-C., Lau, K.-M., Chan, A.K.N., Lau, C.B.-S., and Shing, T.K.M. (2017). Concise and Stereodivergent Synthesis of Carbasugars Reveals Unexpected Structure-Activity Relationship (SAR) of SGLT2 Inhibition. Sci. Rep. 7, 5581. 10.1038/s41598-017-05895-9.
  1. Wong, W.K.R., Fu, Z., Wang, Y.Y., Ng, K.L., and Chan, A.K.N. Engineering of Efficient Escherichia coli Excretion Systems for the Production of Heterologous Proteins for Commercial Applications. Recent Patents on Chem. Eng. 5, 45–55. 10.2174/2211334711205010045.
  1. Chan, A.K.N.*, Wang, Y.Y., Ng, K.L., Fu, Z., and Wong, W.K.R. (2012). Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known β-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea. Gene 493, 52–61. 10.1016/j.gene.2011.11.027.
  1. Wang, Y.Y., Fu, Z.B., Ng, K.L., Lam, C.C., Chan, A.K.N., Sze, K.F., and Wong, W.K.R. (2011). Enhancement of excretory production of an exoglucanase from Escherichia coli with phage shock protein A (PspA) overexpression. J. Microbiol. Biotechnol. 21, 637–645. 10.4014/jmb.1101.01036.
  1. Wang, Y.Y., Ng, K.L., Lam, C.C., Chan, K.N., Sze, K.F., Fu, Z.B., and Wong, W.K.R. (2010). Efficient Bacillus subtilis Promoters for Graded Expression of Heterologous Genes in Escherichia coli. Res. J. Biotechnol. 5, 5–14. link.

Selected Conferences (8)

Reverse Chronological Order

8. Zhang, L., Li, M., Chan, A.K.N., Liu, Q., Kang, H., Pokharel, S.P., Mattson, N., Singh, P., Yang, L., and Chen, C.-W.D. (2023). COX4I1 Controls Mitochondrial Electron Transport Chain Complex IV Assembly and Leukemia Progression in Acute Myeloid Leukemia. Blood 142, 581. 10.1182/blood-2023-188526.

7. Mattson, N., Miyashita, K., Chan, A., Yang, L., Pokharel, S.P., Liu, W., Li, M., Liu, Q., Xu, X., Singh, P., et al. (2023). Abstract 6234: Targeting integrin alpha V beta 5 heterodimer stability using a novel small molecular inhibitor for tumor suppression. Cancer Res. 83, 6234. 10.1158/1538-7445.AM2023-6234.

6. Xu, X., Yang, L., Chan, A., Pokharel, S.P., Li, M., Liu, Q., Mattson, N., and Chen, C. (2022). Abstract 2967: ACTR5 maintains hepatocellular carcinoma via H3K9 epigenetic silencing of CDKN2A. Cancer Res. 82, 2967. 10.1158/1538-7445.AM2022-2967.

5. Mattson, N., Miyashita, K., Chan, A., Yang, L., Pokharel, S.P., Lu, W., Li, M., Liu, Q., Xu, X., Feng, M., et al. (2022). Abstract 2920: Targeting integrin alpha V beta 5 heterodimer stability using a novel small molecular inhibitor for tumor suppression. Cancer Res. 82, 2920. 10.1158/1538-7445.AM2022-2920.

4. Li, M., Yang, L., Pokharel, S., Chan, A., Mattson, N., Liu, Q., Xu, X., and Chen, C.-W. (2022). Abstract 1467: RUVBL1 controls protein synthesis and tumor progression via MYC-dependent EEF1A1 expression. Cancer Res. 82, 1467. 10.1158/1538-7445.AM2022-1467.

3. Qin, X., Su, R., Yang, L., Chan, A.K.N., Deng, X., Qing, Y., Klemm, L., Müschen, M., Chen, C.-W.D., and Chen, J. (2019). Identification of ZNF217 As an Essential Oncogenic Gene in B-Cell Acute Lymphoblastic Leukemia By CRISPR/Cas9-Based Library Screening. Blood 134, 1465. 10.1182/blood-2019-129849.

2. Chan, A.K.N., Delaney, C.D., Yang, L., Chang, W.-H., Miyashita, K., Liu, Q., Pokharel, S.P., Armstrong, S.A., and Chen, C.-W.D. (2019). Tudor Domain-Focused CRISPR Dropout Screen Identifies SGF29 As a Novel Essential Gene in MLL-Rearranged Leukemias. Blood 134, 530. 10.1182/blood-2019-131467. (Talk; 61st ASH Abstract Achievement Award to Chan, A.K.N.)

1. Chen, C.-W., Yang, L., Wang, X., Chan, A., Delaney, C., Miyashita, K., Guo, Y., Chang, W.-H., Li, S., Pokharel, S., et al. (2018). High-Density CRISPR Scan Identifies Functional Regions of DOT1L That Mediate Therapeutic Response in MLL-r Leukemia. Blood 132, 179. 10.1182/blood-2018-179.